pEgr-P16重组质粒的构建及其在EC9706细胞中的辐射诱导表达

doi:10.3969/j.issn.1004-616x.2003.04.004

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2003, Vol. 15 ›› Issue (4): 203-204.doi: 10.3969/j.issn.1004-616x.2003.04.004

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CONSTRUCTION OF pEgr-P16 PLASMID AND ITS EXPRESSION IN EC9706 CELL INDUCED BY IONIZING IRRADIATION

WU Cong-Mei1, HUANG Tian-Hua1，WU De-Sheng, et al

1. Research Center of Reproductive Medicine, Shantou University Medica College, Shantou 515031, China; 2. Department of forensic medicine, Shantou University Medical College, Shantou 515031, China

Received:2003-06-30 Revised:2003-07-16 Online:2003-10-30 Published:2003-10-30
Contact: HUANG Tian-Hua

Abstract

Abstract: Purpose: To construct pEgr-P16 plasmid and detect its expression in esophageal squamous EC9706 cells induced by irradiation. Methods: Human P16 cDNA was ligated to downstream of Egr-1 promoter to construct pEgr-P16 plasmid. The recombined plasmids were transfected into EC9706 cells with liposome. The expression of P16 after different doses of γ-ray irradiation was detected by Westernblot technique. Results: Restriction enzyme digestion showed pEgr-P16 was correctly constructed. The P16 expression in cells transfected with pEgr-P16 induced by different doses of irradiation was higher than that of sham-irradiation group. Conclusion: γ-ray can induce and enhance expression of pEgr-P16 plasmid in EC9706 cells.

Key words: EGR-1 promoter, γ-ray, P16 expression, Western blot, esophageal squamous cell line

WU Cong-Mei, HUANG Tian-Hua, WU De-Sheng, et al. CONSTRUCTION OF pEgr-P16 PLASMID AND ITS EXPRESSION IN EC9706 CELL INDUCED BY IONIZING IRRADIATION[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2003, 15(4): 203-204.

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CONSTRUCTION OF pEgr-P16 PLASMID AND ITS EXPRESSION IN EC9706 CELL INDUCED BY IONIZING IRRADIATION

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